PMC

Active oxygen consumption (AOC) is the difference between aerobic oxygen consumption and routine oxygen consumption during swimming period; excess post-exercise oxygen consumption (EPOC) is the difference between aerobic oxygen consumption and routine oxygen consumption during recovery from exhaustion.

Characteristics of C3H10T1/2/Nluc spheroids after the measurement of oxygen consumption rate.
(A) The luciferase activity of C3H10T1/2/Nluc spheroids after measurement of the oxygen consumption rate. Before and after groups indicate the luciferase activity before or after measurement of the oxygen consumption rate using the OECD, respectively. Representative of two independent experiments with similar results is shown. Results are expressed as the mean ± SD of five samples. ns, not significant. (B) The luciferase activity of secreted from C3H10T1/2/Nluc spheroids after measurement of oxygen consumption rate. Before and after groups indicate the luciferase activity before or after measurement of the oxygen consumption rate using the OECD, respectively. Representative of two independent experiments with similar results is shown. Results are expressed as the mean ± SD of twenty samples. ns, not significant. (C) The proliferation of C3H10T1/2/Nluc spheroids after measurement of the oxygen consumption rate. Before and after groups indicate the proliferation before or after measurement of oxygen consumption rate using OECD, respectively. Representative of two independent experiments with similar results is shown. Results are expressed as the mean ± SD of five samples. ns, not significant. (D) Typical images of C3H10T1/2/Nluc spheroids after 24 h of measurement of oxygen consumption rate. Before and after groups indicate the images before and after measurement of oxygen consumption rate using OECD, respectively. Representative of five independent experiments with similar results is shown. Scale bars represent 100 μm.

Values are means ±s.e.m. Systemic oxygen consumption (VO₂), hepatic oxygen consumption, hepatic arterial oxygen consumption (HaVO₂), portal oxygen consumption, splanchnic oxygen consumption, renal oxygen consumption, and renal and hepatic tissue oxygenation by near-infrared spectroscopy (NIR). P values represent the ANOVA test. * Significantly different from baseline, P < 0·05.

Endogenous oxygen consumption (A), basal_CI+II oxygen consumption (B), maximal uncoupled oxygen consumption following addition of FCCP (C), level of mitochondrial uncoupling (D) and maximal coupled/basal_CI+II ratio (E), measured using the Oxygraph-2k. Endogenous oxygen consumption and basal_CI+II oxygen consumption (following addition of complex I and II substrates) show subtle, but not significant, increased rates for R6/2 inguinal WAT compared to WT. Maximal uncoupled oxygen consumption following FCCP addition was comparable, however, for both genotypes. Mitochondrial uncoupling, measured as the ratio of basal_CI+II /maximal uncoupled oxygen consumption was significantly increased in R6/2 WAT, suggesting that the mitochondria display an increased uncoupling activity. This finding was further confirmed by a reduced maximal coupled/basal_CI+II ratio in R6/2 inguinal WAT. Oxygen consumption rates are expressed per mg WAT. All data are represented as means ± SEM of seven independent experiments, * P < 0.05.